Protein mixture. This would result in any substance

Protein purification refers to processes that serve to single
out or isolate proteins from a mixture, whether this is a single protein or
multiple proteins. The importance of this process is that it is needed in order
to properly characterize aspects of the protein in interest – the protein’s
function, its interactions with other materials in the mixture, and its structure
1. Protein purification can be used to separate proteins from
non-proteins in a mixture or to separate proteins from other proteins in a
mixture. In this case, the process used for protein purification was a resin
column with the element Nickel bound in the middle of the column to obtain only
the proteins and remove non-protein elements of the mixture. This would result
in any substance without histidine, which is an ?-amino acid that is used for
protein biosynthesis
2, not binding to the Nickel and flowing through the column. In
order to remove the protein from the column, it would require another step in
protein purification that would have to bind even tighter to the histidine than
Nickel; this would be Imidazole, our elution buffer. Imidazole has its ring
structure incorporated into histidine and is therefore able to compete with it
for binding sites on Nickel, allowing this solution to elute the protein from
the column 3. In this experiment, the sample loaded into the
column was a green fluorescent protein (GFP) without a linker 3.